Integrated Sustainability Score Implementation as an Objective Function in Sustainable Metabolic Engineering

The sustainable metabolic engineering (SME) concept was defined by Stalidzans and Dace as an approach to the selection of most designs taking economic, environmental social components sustainability into account. At centre calculations is a genome-scale model that provides full balance all incoming outgoing fluxes at steady state. Therefore, indicators are assigned for each exchange reaction, enabling calculation features consumption or production metabolite. further development SME depends on its implementation computational level acquire applicable results—sustainable strain designs. This study proposes first time workflow tools using constraint-based stoichiometric modelling, models growth-coupled product synthesis approach. To demonstrate application SME, relatively simple task has been carried out. have identified Escherichia coli chassis organism, glucose substrate gene deletions tool. A design tool used reduce variability sustainability. 10,000 found produce succinate main with number deleted genes ranging from two seven. Many similar were due combinatorial explosion different alternative combinations deletion sets same impact metabolism.